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An Introduction to Genetic Analysis

An Introduction to Genetic Analysis
   Added On :  03.06.2016 05:01 am
   Author :  Anthony J.F. Griffiths, Susan R. Wessler,
   For :  Genetic

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An Introduction to Genetic Analysis : Eighth Edition - Anthony J.F. Griffiths (U. of British Columbia), Susan R. Wessler (U. of Georgia), Richard C. Lewontin (Harvard U.), William M. Gelbart (Harvard U.), David T. Suzuki (U. of British Columbia), Jeffrey H. Miller (U. of California, Los Angeles).



1. Genetics and the organism Contains a new section introducing model organisms 1.1 Genes as determinants of the inherent properties of species 1.2 Genetic variation 1.3 Methodologies used in genetics 1.4 Genes, the environment, and the organism PART I: TRANSMISSION GENETIC ANALYSIS 2. Patterns of Inheritance All patterns of inheritance are now in one chapterincluding extranuclear inheritance 2.1 Autosomal inheritance 2.2 Sex chromosomes and sex-linked inheritance 2.3 Cytoplasmic Inheritance 3. Chromosomal basis of inheritance A new chapter organization builds concepts logically and clearly. For pedagogical unity, the material on chromosome segregation in tetrad analysis of haploids has been relocated to this chapter. Description of all chromosome types, including organelle chromosomes, is now in one chapter. 3.1 Historical development of the chromosome theory 3.2 The nature of chromosomes 3.3 Mitosis and meiosis 3.4 Chromosome behavior and inheritance patterns in eukaryotes 3.5 Organelle chromosomes 4. Eukaryote Chromosome Mapping by Recombination All mapping is now under one roof with the inclusion of mapping by tetrad analysis and mapping functions in this chapter. The former special eukaryotic mapping chapter is eliminated. 4.1 The discovery of the inheritance patterns of linked genes 4.2 Recombination 4.3 Linkage maps 4.4 Using the chi-square test in linkage analysis 4.5 Using Lod scores to assess linkage in human pedigrees 4.6 Accounting for unseen multiple crossovers 5. The Genetics of Bacteria and Their Viruses Minor streamlining and reorganization to improve accessibility. 5.1 Working with microorganisms 5.2 Bacterial conjugation 5.3 Bacterial transformation 5.4 Bacteriophage genetics 5.5 Transduction 5.6 Physical maps versus linkage maps PART II: THE RELATIONSHIP OF DNA AND PHENOTYPE 6. Gene to Phenotype. A new slant focuses this chapter (formerly called Gene Interaction) on the molecular steps between genes and the phenotype. The work of Beadle and Tatum is relocated to this chapter to make a transition from the transmission genetics of chapters 2, 3 and 4 into molecular genetics from here on. To make this change of gear work, the chapter has been moved so that it is now Chapter 6 instead of Chapter 4. 6.1 Genes and gene products 6.2 Interactions between the alleles of one gene 6.3 Interacting genes and proteins 6.4 Application of chi-square (?2) test to gene interaction ratios 7. Structure and Replication of DNA Introduces the concept of molecular machines, focusing on the replisome. Focus on molecular machines makes it easier for students to understand that replication requires the integration of many events to ensure speed and accuracy. Thorough rewriting and updating of the mechanism of replication Stronger focus on the differences between prokaryotic and eukaryotic replication, with expanded coverage of eukaryotic processes. 7.1 DNA: the genetic material 7.2 The DNA structure 7.3 Semiconservative replication 7.4 Overview of DNA replication 7.5 The replisome: A remarkable replication machine 7.6 Assembling the replisome: replication initiation 7.7 Telomeres and telomerase: replication termination 8. RNA: Transcription and Processing Completely rewritten, updated, and expanded description of transcription in eukaryotes. Better contrasts the complexities of transcription initiation in eukaryotes with events in prokaryotes. RNA polymerase is now presented as a biological machine in order to better integrate transcription and processing events in eukaryotes. A brief discussion of self-splicing introns introduces the concept of the RNA world ? a thread picked up in chapter 9. 8.1 RNA 8.2 Transcription 8.3 Transcription in eukaryotes 9. Proteins and their Translation For unity, the coverage of proteins has been combined into a single chapter, including a brief introduction to proteins and Yanofsky's proof of gene and protein colinearity from former chapter on "genetics of gene function," now eliminated. Thoroughly rewritten and updated description of translation to convey the excitement surrounding the first molecular level glimpses of the ribosome and its interactions with mRNA and tRNAs during protein synthesis. 9.1 Protein structure 9.2 Colinearity of gene and protein 9.3 The genetic code 9.4 tRNA: the adaptor 9.5 Ribosomes 9.6 Posttranslational events 10. Regulation of Gene Transcription An all-new and expanded treatment of transcription regulation in eukaryotes emphasizes the role of chromatin and epigenetic mechanisms. Topics covered include: puzzling epigenetic mechanisms such as imprinting, position effect variegation and X-chromosome inactivation the role of yeast genetics in identifying the components of the epigenetic machinery. the histone code and the efforts of scientists to understand how it leads to alterations in chromatin condensation and gene expression. 10.1 Prokaryotic gene regulation 10.2 Discovery of the lac system of negative control 10.3 Catabolite repression of the lac operon: positive control 10.4 Dual positive and negative control: the arabinose operon 10.5 Metabolic Pathways 10.6 Transcriptional regulation in eukaryotes 10.7 The involvement of chromatin in eukaryotic gene regulation PART III: GENOME STRUCTURE AND ENGINEERING 11. Gene Isolation and Manipulation Combines coverage of recombinant DNA technology in a single chapter through streamlining and elimination of excessive detail. Includes new material on genetic engineering using specific model organisms. 11.1 Generating recombinant molecules 11.2 DNA amplification in vitro: the polymerase chain reaction 11.3 Zeroing in on the gene for alkaptonuria: another case study 11.4 Detecting human disease alleles: molecular genetic diagnostics 11.5 Genetic engineering 12. Genomics Entirely reconceived, and extensively rewritten and updated, to provide a clear, accessible view of the strategies used to sequence genomes and to locate genes within genome sequences. Includes new section on bioinformatics describes how scientists are analyzing the information content of the genome. Includes new section discussing what genome sequencing has told us about the structure of the human genome. 12.1 The nature of genomics 12.2 The sequence map of a genome 12.3 Creating genomic sequence maps 12.4 Using genomic sequence to find a specific gene 12.5 Bioinformatics: meaning from genomic sequence 12.6 Take-home lessons from the genomes 12.7 Functional genomics 13. The Dynamic Genome: Transposable Elements For unity, almost everything related to transposable elements has been combined into one chapter. The story moves into the genomics age with the remarkable discovery from the human genome project that more than half of our genome is derived from transposable elements. New sections describe how transposable elements have evolved diverse strategies to thrive in the genomes of a variety of organisms without harming their host. 13.1 Discovery of transposable elements in maize 13.2 Transposable elements in prokaryotes 13.3 Transposable elements in eukaryotes 13.4 The dynamic genome: more transposable elements than ever imagined 13.5 Host regulation of transposable elements PART IV: THE NATURE OF HERITABLE CHANGE 14: Mutation, Repair, and Recombination Two chapters in the seventh edition on mutation and recombination have been combined into a clear and concise description of the two major processes responsible for genetic variation. Simplified and Streamlined descriptions of repair systems A new section on the repair of mutagenic double strand breaks provides a logical entry point for the discussion of recombination. 14.1 Point mutations 14.2 Spontaneous mutation 14.3 Biological repair mechanisms 14.4 The mechanism of meiotic crossing-over 15. Large-Scale Chromosomal Changes Two chapters in the seventh edition have been merged to make the topic less onerous and more streamlined. 15.1 Changes in chromosome number 15.2 Changes in chromosome structure 15.3 Overall incidence of human chromosome mutations PART V: FROM GENES TO PROCESSES 16. Dissection of gene function A new chapter in IGA, Chapter 16 describes the strategies used for forward and reverse genetics, including cutting-edge techniques such as RNAi and other types of phenocopying. Includes examples of screens used with several model organisms. 16.1 Forward genetics 16.2 Reverse genetics 16.3 Analysis of recovered mutations 16.4 Broader applications of functional dissection 17. Genetic Regulation of Cell Number: Normal and Cancer Cells Revised to achieve a smoother, more easily followed flow from concept to concept. Includes a new section on applying genomic approaches to cancer research, diagnosis, and therapies. 17.1 The balance between cell loss and proliferation 17.2 The cell proliferation machinery of the cell cycle 17.3 The machinery of programmed cell death 17.4 Extracellular signals 17.5 Cancer: the genetics of aberrant cell number regulation 17.6 Applying genomic approaches to cancer research, diagnosis and therapies 18. The Genetic Basis of Development Includes new sections on sex determination in humans and genomic approaches to understanding pattern formation. 18.1 The logic of building the body plan 18.2 Binary fate decisions: the germ line versus the soma 18.3 Forming complex pattern: the logic of the decision-making process 18.4 Forming complex pattern: establishing positional information 18.5 Forming complex pattern: utilizing positional information to establish cell fates 18.6 Refining the pattern 18.7 The many parallels in vertebrate and insect pattern formation 18.8 The genetics of sex determination in humans 18.9 Do the lessons of animal development apply to plants? 18.10 Genomic approaches to understanding pattern formation PART VI: THE IMPACT OF GENETIC VARIATION 19. Population Genetics 19.1 Variation and its modulation 19.2 Effect of sexual reproduction on variation 19.3 Sources of variation 19.4 Balanced polymorphism 19.5 Random events 20. Quantitative Genetics 20.1 Genes and quantitative traits 20.2 Some basic statistical notions 20.3 Genotypes and phenotypic distribution 20.4 Determining norms of reaction 20.5 The heritability of a quantitative character 20.6 Quantifying heritability 20.7 Locating genes 21. Evolutionary Genetics Includes new sections on genetic evidence of common ancestry in evolution and on comparative genomics and proteomics 21.1 A synthesis of forces: variation and divergence of populations 21.2 Multiple adaptive peaks 21.3 Heritability of variation 21.4 Observed variation within and between populations 21.5 The process of speciation 21.6 Origin of new genes 21.7 Rate of molecular evolution 21.8 Genetic evidence of common ancestry in evolution 21.9 Comparative genomics and proteomics

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